Clinical Proteomics:Article Title: Differential localization of A-Raf regulates MST2-mediated apoptosis during epithelial differentiation
Article Snippet: Immunblot analysis Protein lysates or immunoprecipitates were resolved by SDS-PAGE (10–15%) and blotted on polyvinylidene difluoride membrane (Millipore, Darmstadt, Germany). .. Protein visualization was performed using the following antibodies in combination with horseradish peroxidase-conjugated secondary antibodies and the enhanced chemiluminescence system (GE Healthcare, Little Chalfont, UK): polyclonal goat anti-human A-Raf antibody sc-30703 (Santa Cruz Biotechnology), monoclonal mouse anti-human A-Raf antibody (12C9, sc-100420; Santa Cruz Biotechnology), polyclonal rabbit anti-human MST2 antibody (Stratagene, San Diego, CA, USA), monoclonal rabbit anti-human MST2 (N terminus) antibody (Epitomics, Burlingame, CA, USA), monoclonal mouse anti-flag antibody M2 (Sigma-Aldrich, St Louis, MO, USA), monoclonal mouse anti-human tubulin antibody (Santa Cruz Biotechnology), monoclonal mouse anti-human alpha 1 sodium potassium ATPase (ATP1A1) antibody (plasma membrane marker; Abcam, Cambridge, UK), polyclonal rabbit anti-human MEK1/2 antibody (12-B, sc-436; Santa Cruz Biotechnology), monoclonal rabbit anti-human phosphorylated Mst1 (pT183)/Mst2 (pT180; Epitomics), monoclonal mouse anti-HA tag antibody 3F10 (Roche Diagnostics, Risch-Rotkreuz, Switzerland), polyclonal rabbit anti-human Puma antibody (Sigma-Aldrich), polyclonal rabbit anti-human caspase-3 antibody (Santa Cruz Biotechnology), monoclonal rabbit anti-human caspase-3 antibody (8G10; Santa Cruz Biotechnology), monoclonal mouse anti-human PARP antibody (Becton Dickinson, Franklin Lakes, NJ, USA), polyclonal rabbit anti-human hnRNP H antibody, polyclonal rabbit anti-human MAPK (ERK1 and ERK2) antibody (Sigma-Aldrich), monoclonal mouse anti-human MAPK-activated (diphosphorylated ERK1 and ERK2) antibody (Sigma-Aldrich), monoclonal rabbit anti-GFP antibody (D5.1; Cell Signaling Technology, Danvers, MA, USA), monoclonal rabbit anti-human KSR1 antibody (Epitomics), mouse monoclonal anti-human KSR2 antibody (K75, sc-100421; Santa Cruz Biotechnology), polyclonal rabbit anti-human AKT antibody (Cell Signaling Technology), polyclonal rabbit anti-human activated Akt (phosphorylated at Ser473) antibody (Cell Signaling Technology), and monoclonal mouse anti-human caspase 8 (1C12; Cell Signaling Technology). .. Immunoprecipitation Immunoprecipitations were conducted as described previously 21 with the following immobilized antibodies: monoclonal mouse anti-HA tag antibody 3F10 (Roche Diagnostics), monoclonal mouse anti-flag antibody M2 (Sigma-Aldrich), polyclonal goat anti-human MST2 antibody sc-6211 (Santa Cruz Biotechnology), polyclonal goat anti-human A-Raf antibody sc-30703 (Santa Cruz Biotechnology), and monoclonal mouse anti-V5-tag antibody (Invitrogen).
Membrane:Article Title: Differential localization of A-Raf regulates MST2-mediated apoptosis during epithelial differentiation
Article Snippet: Immunblot analysis Protein lysates or immunoprecipitates were resolved by SDS-PAGE (10–15%) and blotted on polyvinylidene difluoride membrane (Millipore, Darmstadt, Germany). .. Protein visualization was performed using the following antibodies in combination with horseradish peroxidase-conjugated secondary antibodies and the enhanced chemiluminescence system (GE Healthcare, Little Chalfont, UK): polyclonal goat anti-human A-Raf antibody sc-30703 (Santa Cruz Biotechnology), monoclonal mouse anti-human A-Raf antibody (12C9, sc-100420; Santa Cruz Biotechnology), polyclonal rabbit anti-human MST2 antibody (Stratagene, San Diego, CA, USA), monoclonal rabbit anti-human MST2 (N terminus) antibody (Epitomics, Burlingame, CA, USA), monoclonal mouse anti-flag antibody M2 (Sigma-Aldrich, St Louis, MO, USA), monoclonal mouse anti-human tubulin antibody (Santa Cruz Biotechnology), monoclonal mouse anti-human alpha 1 sodium potassium ATPase (ATP1A1) antibody (plasma membrane marker; Abcam, Cambridge, UK), polyclonal rabbit anti-human MEK1/2 antibody (12-B, sc-436; Santa Cruz Biotechnology), monoclonal rabbit anti-human phosphorylated Mst1 (pT183)/Mst2 (pT180; Epitomics), monoclonal mouse anti-HA tag antibody 3F10 (Roche Diagnostics, Risch-Rotkreuz, Switzerland), polyclonal rabbit anti-human Puma antibody (Sigma-Aldrich), polyclonal rabbit anti-human caspase-3 antibody (Santa Cruz Biotechnology), monoclonal rabbit anti-human caspase-3 antibody (8G10; Santa Cruz Biotechnology), monoclonal mouse anti-human PARP antibody (Becton Dickinson, Franklin Lakes, NJ, USA), polyclonal rabbit anti-human hnRNP H antibody, polyclonal rabbit anti-human MAPK (ERK1 and ERK2) antibody (Sigma-Aldrich), monoclonal mouse anti-human MAPK-activated (diphosphorylated ERK1 and ERK2) antibody (Sigma-Aldrich), monoclonal rabbit anti-GFP antibody (D5.1; Cell Signaling Technology, Danvers, MA, USA), monoclonal rabbit anti-human KSR1 antibody (Epitomics), mouse monoclonal anti-human KSR2 antibody (K75, sc-100421; Santa Cruz Biotechnology), polyclonal rabbit anti-human AKT antibody (Cell Signaling Technology), polyclonal rabbit anti-human activated Akt (phosphorylated at Ser473) antibody (Cell Signaling Technology), and monoclonal mouse anti-human caspase 8 (1C12; Cell Signaling Technology). .. Immunoprecipitation Immunoprecipitations were conducted as described previously 21 with the following immobilized antibodies: monoclonal mouse anti-HA tag antibody 3F10 (Roche Diagnostics), monoclonal mouse anti-flag antibody M2 (Sigma-Aldrich), polyclonal goat anti-human MST2 antibody sc-6211 (Santa Cruz Biotechnology), polyclonal goat anti-human A-Raf antibody sc-30703 (Santa Cruz Biotechnology), and monoclonal mouse anti-V5-tag antibody (Invitrogen).
Marker:Article Title: Differential localization of A-Raf regulates MST2-mediated apoptosis during epithelial differentiation
Article Snippet: Immunblot analysis Protein lysates or immunoprecipitates were resolved by SDS-PAGE (10–15%) and blotted on polyvinylidene difluoride membrane (Millipore, Darmstadt, Germany). .. Protein visualization was performed using the following antibodies in combination with horseradish peroxidase-conjugated secondary antibodies and the enhanced chemiluminescence system (GE Healthcare, Little Chalfont, UK): polyclonal goat anti-human A-Raf antibody sc-30703 (Santa Cruz Biotechnology), monoclonal mouse anti-human A-Raf antibody (12C9, sc-100420; Santa Cruz Biotechnology), polyclonal rabbit anti-human MST2 antibody (Stratagene, San Diego, CA, USA), monoclonal rabbit anti-human MST2 (N terminus) antibody (Epitomics, Burlingame, CA, USA), monoclonal mouse anti-flag antibody M2 (Sigma-Aldrich, St Louis, MO, USA), monoclonal mouse anti-human tubulin antibody (Santa Cruz Biotechnology), monoclonal mouse anti-human alpha 1 sodium potassium ATPase (ATP1A1) antibody (plasma membrane marker; Abcam, Cambridge, UK), polyclonal rabbit anti-human MEK1/2 antibody (12-B, sc-436; Santa Cruz Biotechnology), monoclonal rabbit anti-human phosphorylated Mst1 (pT183)/Mst2 (pT180; Epitomics), monoclonal mouse anti-HA tag antibody 3F10 (Roche Diagnostics, Risch-Rotkreuz, Switzerland), polyclonal rabbit anti-human Puma antibody (Sigma-Aldrich), polyclonal rabbit anti-human caspase-3 antibody (Santa Cruz Biotechnology), monoclonal rabbit anti-human caspase-3 antibody (8G10; Santa Cruz Biotechnology), monoclonal mouse anti-human PARP antibody (Becton Dickinson, Franklin Lakes, NJ, USA), polyclonal rabbit anti-human hnRNP H antibody, polyclonal rabbit anti-human MAPK (ERK1 and ERK2) antibody (Sigma-Aldrich), monoclonal mouse anti-human MAPK-activated (diphosphorylated ERK1 and ERK2) antibody (Sigma-Aldrich), monoclonal rabbit anti-GFP antibody (D5.1; Cell Signaling Technology, Danvers, MA, USA), monoclonal rabbit anti-human KSR1 antibody (Epitomics), mouse monoclonal anti-human KSR2 antibody (K75, sc-100421; Santa Cruz Biotechnology), polyclonal rabbit anti-human AKT antibody (Cell Signaling Technology), polyclonal rabbit anti-human activated Akt (phosphorylated at Ser473) antibody (Cell Signaling Technology), and monoclonal mouse anti-human caspase 8 (1C12; Cell Signaling Technology). .. Immunoprecipitation Immunoprecipitations were conducted as described previously 21 with the following immobilized antibodies: monoclonal mouse anti-HA tag antibody 3F10 (Roche Diagnostics), monoclonal mouse anti-flag antibody M2 (Sigma-Aldrich), polyclonal goat anti-human MST2 antibody sc-6211 (Santa Cruz Biotechnology), polyclonal goat anti-human A-Raf antibody sc-30703 (Santa Cruz Biotechnology), and monoclonal mouse anti-V5-tag antibody (Invitrogen).
Immunoprecipitation:Article Title: Differential localization of A-Raf regulates MST2-mediated apoptosis during epithelial differentiation
Article Snippet: Protein visualization was performed using the following antibodies in combination with horseradish peroxidase-conjugated secondary antibodies and the enhanced chemiluminescence system (GE Healthcare, Little Chalfont, UK): polyclonal goat anti-human A-Raf antibody sc-30703 (Santa Cruz Biotechnology), monoclonal mouse anti-human A-Raf antibody (12C9, sc-100420; Santa Cruz Biotechnology), polyclonal rabbit anti-human MST2 antibody (Stratagene, San Diego, CA, USA), monoclonal rabbit anti-human MST2 (N terminus) antibody (Epitomics, Burlingame, CA, USA), monoclonal mouse anti-flag antibody M2 (Sigma-Aldrich, St Louis, MO, USA), monoclonal mouse anti-human tubulin antibody (Santa Cruz Biotechnology), monoclonal mouse anti-human alpha 1 sodium potassium ATPase (ATP1A1) antibody (plasma membrane marker; Abcam, Cambridge, UK), polyclonal rabbit anti-human MEK1/2 antibody (12-B, sc-436; Santa Cruz Biotechnology), monoclonal rabbit anti-human phosphorylated Mst1 (pT183)/Mst2 (pT180; Epitomics), monoclonal mouse anti-HA tag antibody 3F10 (Roche Diagnostics, Risch-Rotkreuz, Switzerland), polyclonal rabbit anti-human Puma antibody (Sigma-Aldrich), polyclonal rabbit anti-human caspase-3 antibody (Santa Cruz Biotechnology), monoclonal rabbit anti-human caspase-3 antibody (8G10; Santa Cruz Biotechnology), monoclonal mouse anti-human PARP antibody (Becton Dickinson, Franklin Lakes, NJ, USA), polyclonal rabbit anti-human hnRNP H antibody, polyclonal rabbit anti-human MAPK (ERK1 and ERK2) antibody (Sigma-Aldrich), monoclonal mouse anti-human MAPK-activated (diphosphorylated ERK1 and ERK2) antibody (Sigma-Aldrich), monoclonal rabbit anti-GFP antibody (D5.1; Cell Signaling Technology, Danvers, MA, USA), monoclonal rabbit anti-human KSR1 antibody (Epitomics), mouse monoclonal anti-human KSR2 antibody (K75, sc-100421; Santa Cruz Biotechnology), polyclonal rabbit anti-human AKT antibody (Cell Signaling Technology), polyclonal rabbit anti-human activated Akt (phosphorylated at Ser473) antibody (Cell Signaling Technology), and monoclonal mouse anti-human caspase 8 (1C12; Cell Signaling Technology). .. Immunoprecipitation Immunoprecipitations were conducted as described previously 21 with the following immobilized antibodies: monoclonal mouse anti-HA tag antibody 3F10 (Roche Diagnostics), monoclonal mouse anti-flag antibody M2 (Sigma-Aldrich), polyclonal goat anti-human MST2 antibody sc-6211 (Santa Cruz Biotechnology), polyclonal goat anti-human A-Raf antibody sc-30703 (Santa Cruz Biotechnology), and monoclonal mouse anti-V5-tag antibody (Invitrogen). ..
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